FastQCFastQC Report
Tue 14 May 2024
EGAF00007947068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78894290
Total Bases11.9 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG5344590.677436858865198Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2088800.2647593380965847No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATCGTAA987160.12512439113147478TruSeq Adapter, Index 7 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATCGTGA821620.10414188403241859TruSeq Adapter, Index 7 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)