FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68180504
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG13772642.0200261353304163Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAA5083700.7456237049817056TruSeq Adapter, Index 25 (97% over 38bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAAC4959120.7273516194600146TruSeq Adapter, Index 25 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAT4259480.6247357749071494TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAAT4128520.6055279380158293TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAAA4027920.5907729869523991TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACATA3950720.5794501020409002TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACACT3814480.5594678502229904TruSeq Adapter, Index 25 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAC3725430.546406931811475TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACACA3304160.48461947421215895TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAAC3265460.4789433648070422TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACATC3136500.4600288669030666TruSeq Adapter, Index 25 (97% over 38bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACA3040900.4460072633079978TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACATT2802470.4110368559317191TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACACC2187460.32083365062833796TruSeq Adapter, Index 25 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAG1821460.26715261594428813TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAGC1819170.2668167428037786TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAAG1653080.24245640659975176TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAGT1509280.2213653334096797TruSeq Adapter, Index 25 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1424710.20896149432981606No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAGA1421640.20851121898424219TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACATG1377660.20206069465253587TruSeq Adapter, Index 25 (97% over 38bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAA1327380.19468615251069427TruSeq Adapter, Index 25 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACACTC1151020.16881952060665317TruSeq Adapter, Index 25 (97% over 38bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGG1076570.15789997680275286TruSeq Adapter, Index 5 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACATCT1067380.15655208415590474TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACACG1024660.1502863633862255TruSeq Adapter, Index 25 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAACT1005500.14747617588746484TruSeq Adapter, Index 25 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACACTT961930.14108578604816413TruSeq Adapter, Index 25 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAAAT898800.1318265409126339TruSeq Adapter, Index 25 (97% over 38bp)
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG892630.130921590136676No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA885570.12988610351134983No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAGG862770.1265420390556221TruSeq Adapter, Index 25 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACATAA856820.12566935556827213TruSeq Adapter, Index 25 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACACTA845440.12400025673028171TruSeq Adapter, Index 25 (97% over 38bp)
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC843320.12368931740369651No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACACAT822830.12068405947835176TruSeq Adapter, Index 25 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACACCT820420.1203305859986016TruSeq Adapter, Index 25 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACATAC816420.11974390802391252TruSeq Adapter, Index 25 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAATT800730.11744266366819464TruSeq Adapter, Index 25 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAAAA795790.11671811636945364TruSeq Adapter, Index 25 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAATA776900.11394752963398452TruSeq Adapter, Index 25 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAGCT773790.11349138750866376TruSeq Adapter, Index 25 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAATC758320.11122241044155379TruSeq Adapter, Index 25 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAAAC743150.10899743422254549TruSeq Adapter, Index 25 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACACAA739040.10839462260355248TruSeq Adapter, Index 25 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACATCA726050.10648938588074973TruSeq Adapter, Index 25 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACATAT709300.10403267186173924TruSeq Adapter, Index 25 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)