FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68180504
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51596767.567670664329499No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG12080331.7718158844939016Illumina Single End PCR Primer 1 (100% over 30bp)
CTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGAGAACGTGTA5956950.8737028403310131Illumina Single End PCR Primer 1 (97% over 34bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGAGAACGTGTAGATC1213010.17791156251939702Illumina Single End PCR Primer 1 (97% over 34bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1043180.15300268240903586No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA949010.13919081618991844No Hit
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGAGAACGTG945620.13869360660636948Illumina Single End PCR Primer 1 (97% over 34bp)
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG769860.11291497639853176No Hit
CGTATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGAGAACGT726330.10653045333897795Illumina Single End PCR Primer 1 (97% over 34bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGAGAAC713410.10463548348073226Illumina Single End PCR Primer 1 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)