FastQCFastQC Report
Tue 14 May 2024
EGAF00007947099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69778292
Total Bases10.5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1972930.28274266157159594No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG1851350.265318904624378No Hit
GGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTGGACTGGTGTAG1645790.23585988605166774Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1203540.17248057605078668Illumina Single End PCR Primer 1 (100% over 30bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1178480.16888920124327492No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA738180.10578934778168544No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA722320.10351643459544696No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)