FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45716962
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGAAA3875620.847742244989945TruSeq Adapter, Index 12 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3288090.719227581220292No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCAA3113020.680933260613424TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGAAC2949260.6451128576741385TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGAAT2758120.6033034303547992TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCTA2550190.5578214055430892TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTAA2415310.5283181327753144TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCAC2392260.5232762404465984TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCAT2374630.5194199037110121TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGATA2298020.5026624472553535TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGACA2194390.4799947118095905TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCTC2076120.4541246638392114TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTAC2051780.4488006005298428TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTAT1835390.40146805905431776TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCCA1821370.39840136359017037TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTCA1728210.37802380656877416TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTCT1681150.36773003420481TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGACC1646250.3600961061235871TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCTT1610610.352300312518579TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGAAG1588990.34757121437771826TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCCC1549890.339018590080417TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGAA1548980.3388195392336TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGATT1538610.33655123452866353TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTTA1517970.3320364988382211TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGACT1504210.3290266750445929TruSeq Adapter, Index 12 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG1427870.3123282776313964Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGATC1330590.29104952336946627TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGAGC1301160.2846120877410883TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTGC1296920.2836846420372377TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGAGA1269860.2777656135593612TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGAC1250270.27348055192293835TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGCA1240300.2712997420957237TruSeq Adapter, Index 12 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGAA1224720.2678918166084614TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGCT1220050.26687031391106TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGATG1211760.26505698257027666TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCAG1174960.25700745381987544TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCCT1139380.24922478444652554TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGTA1123540.24575998728874415TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTGA1114010.2436754218270234TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTGT1085170.23736704114328505TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGACG1071650.23440971427628987TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCGC1046890.22899378134531334TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGAT1044420.22845350047538152TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCGA1038010.22705139505989047TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTTT1030330.2253714934076328TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTCC1024780.2241575019792435TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCTG1024750.22415093986341436TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGCC985220.2155042585725622TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGAGT974730.21320970540430922TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTAG967020.21152324163622246TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCGT941530.2059476305534038TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTTC900450.19696190661137983TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGTC836740.1830261599622477TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGAGG823100.18004258463193595TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGTG799730.17493069640104256TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTTG791860.1732092346818671TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGTT761480.1665639987189TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGGC694370.15188454560913298TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTGG643700.14080113197373TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCGG631150.13805598018521004TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGGA586210.12822593067317115TruSeq Adapter, Index 12 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTA535130.11705283478810338TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTCG523200.11444330006005211TruSeq Adapter, Index 12 (97% over 38bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTT513130.11224061651340698TruSeq Adapter, Index 12 (97% over 38bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTC507310.1109675660425555TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGGT501470.1096901408278179TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGAG499090.10916954630537348TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGCG496100.10851552209440339TruSeq Adapter, Index 12 (97% over 38bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTG491030.10740652451928018TruSeq Adapter, Index 12 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCCG467190.10219182980706373TruSeq Adapter, Index 12 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)