FastQCFastQC Report
Thu 16 May 2024
EGAF00007947101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45716962
Total Bases6.9 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4431700.969377623998725No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2832240.6195162311966399No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1304090.28525298772040014Illumina Single End PCR Primer 1 (100% over 30bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAACTTCGTGT505590.11059133806835197Illumina Single End Sequencing Primer (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)