FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50497247
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1533310.30364229558890604Illumina Single End PCR Primer 1 (100% over 30bp)
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG641200.12697721917394822No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG580140.11488547088517517No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)