FastQCFastQC Report
Wed 15 May 2024
EGAF00007947110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54091584
Total Bases8.1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG4234520.7828426692034015Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGT1509170.2790027372834931TruSeq Adapter, Index 9 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGTGC928060.17157197689015724TruSeq Adapter, Index 9 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGTGCA717660.13267498322844457TruSeq Adapter, Index 9 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGTGA688100.1272101774649454TruSeq Adapter, Index 9 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGTGT618090.11426731374699621TruSeq Adapter, Index 9 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGTGCT603320.11153675958167542TruSeq Adapter, Index 9 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGTGAC583470.10786705747052998TruSeq Adapter, Index 9 (97% over 36bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGTG564720.1044007141665513TruSeq Adapter, Index 9 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGTGCC562640.10401618114936327TruSeq Adapter, Index 9 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)