FastQCFastQC Report
Thu 16 May 2024
EGAF00007947112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55184666
Total Bases8.3 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG1567460.2840390480935411Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT730510.13237554069820773No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC700010.12684864306327415No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA654390.11858185387948167No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG586530.10628495966615073No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)