FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65422005
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG3482870.5323698043188986Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3296380.5038641050515037No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTGATTGCCC809550.12374276820161657TruSeq Adapter, Index 25 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTGATTGCAC800190.12231205692946892TruSeq Adapter, Index 25 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTGATTGCCT725660.11091986557122484TruSeq Adapter, Index 25 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTGATTGCTC718330.10979944744891262TruSeq Adapter, Index 25 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTGATTGAA703240.10749288408387972TruSeq Adapter, Index 25 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTGATTGCAA693170.10595364663617388TruSeq Adapter, Index 25 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTGATTGCCA658850.10070770530496581TruSeq Adapter, Index 25 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)