FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56038767
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG7591511.3546889780783364Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4818710.8598886552946463No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTCTC3610440.6442754174088091TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTACT2771850.4946307972836019TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTCT2513380.4485073699069788TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTAC2392220.4268866229694169TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTCTA2284560.4076749226120553TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTGCT2248600.4012579363139806TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTAAC2231370.3981832790860656TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTCTT2204840.39344905643623457TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTCAC2171100.3874282244646817TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTGCC2066190.36870725581810176TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTCCC1999600.3568244105013945TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTACC1955520.348958427297303TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTTC1914490.3416367101724419TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTGCA1786260.31875433661843416TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTCAA1755960.31334736540509533TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTAA1751180.3124943844678096TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTCC1724970.3078172651443241TruSeq Adapter, Index 7 (97% over 35bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT1696100.3026654744205917Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTACA1686830.30101126243552073TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTCCT1678870.29959081719267666TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTTT1664970.2971103914545443TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTCAT1641840.2929828916471342TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTTA1620120.2891070033714339TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTATC1615170.2882236862920271TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTAT1614270.2880630831866804TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTCCA1517960.2708767664356355TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTAAA1512400.26988459614038257TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTAAT1463310.26112458898319446TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTCA1459890.260514297182877TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTGC1376100.24556214807509952TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTATT1352730.24139182077293025TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTATA1241140.22147882018888815TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTGTC1205790.21517068710665957TruSeq Adapter, Index 7 (97% over 35bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG1039450.1854876642806934No Hit
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTT993920.17736293162909883TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTCTG976490.1742525848222178TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTGAC968320.17279466552145945TruSeq Adapter, Index 7 (97% over 35bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC888160.15849028227191364Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA885560.15802631774535653No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTGT841000.15007467955174675TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTGAA838940.14970707688839763TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTGA794310.14174294734214976TruSeq Adapter, Index 7 (97% over 35bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGT762710.1361039938655324TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTTG733550.13090045325229943TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTGTA727710.12985831754649418TruSeq Adapter, Index 7 (97% over 35bp)
CACACACACACACACACACACACACACACACACACACACACACACACACA720190.12851638937737514No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTGAT705340.12586643813915463TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTGTT680670.12146412857370684TruSeq Adapter, Index 7 (97% over 35bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACC676630.12074319907859499TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTATG660210.11781308464549192TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTAGC648400.11570561500755361TruSeq Adapter, Index 7 (97% over 35bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTA628070.11207776930566656TruSeq Adapter, Index 7 (97% over 35bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTAA603810.10774862337709892TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTCGA593860.10597306682354378TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTAAG587270.10479709519661631TruSeq Adapter, Index 7 (97% over 35bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC583640.10414932933838462Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTG570510.10180630847927115TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)