FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64387309
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCAA3975990.6175114415792714TruSeq Adapter, Index 8 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3740940.5810058003821841No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG3291910.5112669019915089Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCTA3181510.4941206659219132TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCAC3045340.4729720883349854TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCTC2580160.40072493167869466TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCAT2530000.3929345765949001TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCCA2508950.3896652987935868TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGACT2409940.3742880448692148TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGACA2271130.3527294485936662TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGAAA2100250.3261900571120312TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCCC2022420.3141022713031849TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCCT2011990.31248238686291424TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCTT1992170.309404140496072TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGAAC1947640.3024881813277831TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGACC1869740.29038952381128397TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGATC1589340.24684056915626026TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGATA1565230.24309604242040928TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGTAC1528710.23742411722782203TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGAAT1491860.23170093969915717TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGTCT1474110.22894418525861984TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGTAA1417450.22014431446420601TruSeq Adapter, Index 8 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGAA1379060.21418195936717901TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGTCA1139170.17692461724095349TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGATT1051750.16334740748367044TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGGCT1043540.16207231148610357TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGGCA1003440.1558443761021291TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGTAT1002880.15575740244090647TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCGA959800.14906664293114036TruSeq Adapter, Index 8 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCA954170.14819224701563471TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGAGC940130.146011693080697TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGGCC923780.14347237279321612TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCTG868160.13483402451250137TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGGAC864840.1343183949495389TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGTTA862250.13391614176638442TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGTCC830180.1289353465602981TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGAGA815030.12658239840400845TruSeq Adapter, Index 8 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGA771500.11982174934504562TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCAG748580.1162620416392926TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGGAT710780.110391319506768TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGGAA699460.10863320906919717TruSeq Adapter, Index 8 (97% over 38bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT688890.10699158121362085Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGTTC673860.10465727027045035TruSeq Adapter, Index 8 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGC671130.10423327367199023TruSeq Adapter, Index 8 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)