FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58302654
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG6365001.0917170254376414Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCAAA2984460.5118909338158087TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCACT2653600.45514223074647686TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCAAT2424620.41586786083528887TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCACA2361770.4050879056037483TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCAAC2276090.39039217665803TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCAA2244850.38503393001629055TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTAA2204090.37804282460280453TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCATA2112760.3623780145583081TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCAA1965300.33708585547409214TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGCA1913430.32818917643097345TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTCT1841320.31582095731010806TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGCT1781300.30552640022184924TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTTA1762470.30229670162185074TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGAA1748450.29989200834665264TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCACC1694820.2906934562532951TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCATC1686140.28920467325552623TruSeq Adapter, Index 1 (97% over 36bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGT1661750.28502133024681864TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTAC1659420.28462169149280925TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCTC1624670.27866141393837746TruSeq Adapter, Index 1 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1598670.27420192569621277No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCATT1530200.2624580349292504TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCTA1489670.2555063788348297TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTCA1449890.2486833618243176TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCAC1441410.24722888258225775TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTAT1432070.24562689719064934TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCAT1401040.24030466949240423TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCCA1384100.23739914138385534TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCAAG1353500.2321506667603845TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTGC1326220.22747163448168245TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTTT1323250.2269622237094044TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTA1258260.21581521829177794TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCCT1258060.21578091453606899TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTGA1235760.21195604577451999TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGAC1223390.20983435848392082TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGTA1215030.2084004614952863TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCAGA1189360.20399757445004135TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCATG1159920.19894806160968248TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGAT1152710.19771141121637448TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGCC1119020.19193294356720023TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCAC1095690.18793141046375006TruSeq Adapter, Index 1 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCA1088890.18676508276964543TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTTC1085860.1862453808706547TruSeq Adapter, Index 1 (97% over 36bp)
AAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAC1080430.18531403390315643TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCAT1076940.1847154333660351TruSeq Adapter, Index 1 (97% over 36bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA1067150.18303626452408153No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGTC1048140.17977569254394493TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCCC1044840.1792096805747471TruSeq Adapter, Index 1 (97% over 36bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTT1004690.17232320161617343TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTGT999940.17150848741808564TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTCC963120.16519316599206615TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCAGC942010.16157240457698546TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCTT936410.16061189941713458TruSeq Adapter, Index 1 (97% over 36bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTC827610.14195065631146053TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCAGT809450.138835875293087TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCA802770.13769012985240775TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGA787890.13513793042766115TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCACG784960.1346353804065249TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTAG759440.13025822117806163TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGTT755730.12962188650966044TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTTG749120.1284881473834793TruSeq Adapter, Index 1 (97% over 36bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCA736120.12625840326239696TruSeq Adapter, Index 1 (96% over 32bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCT728770.12499774024009268TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGC715620.12274226830222858TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGCG708040.121442155960859TruSeq Adapter, Index 1 (97% over 36bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT697530.11963949359835317Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG690430.1184217102706851No Hit
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACC689650.11828792562342015TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGGA688840.11814899541279889TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGTG656470.11259693255130376TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTT654590.11227447724763953TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCGC650640.11159697807238758TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGAG625280.10724726184849148TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCAG622750.10681331933877315TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTCG607710.10423367690945938TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGGC603700.10354588660749474TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)