FastQCFastQC Report
Mon 13 May 2024
EGAF00007947137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62733531
Total Bases9.4 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG3533060.5631852605267826Illumina Single End PCR Primer 1 (100% over 30bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGCAATGTGTGTAGATC3497340.5574913358535486Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2391330.3811884907291445No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)