FastQCFastQC Report
Wed 15 May 2024
EGAF00007947142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49725791
Total Bases7.5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG6046211.2159102707888547Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGA722760.1453491207409853TruSeq Adapter, Index 7 (97% over 37bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGACGT713440.14347484185822204TruSeq Adapter, Index 7 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGACGTGAA706430.14206511063846122TruSeq Adapter, Index 7 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGACGTGA580160.11667184942316956TruSeq Adapter, Index 7 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA509020.10236539022576835No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)