FastQCFastQC Report
Wed 15 May 2024
EGAF00007947143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49725791
Total Bases7.5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG6972801.402250192460488Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1141660.22959111902312426No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCACCAAGTGT601960.12105589230345275Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCAC549680.11054223350614976Illumina Single End Sequencing Primer (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)