FastQCFastQC Report
Thu 16 Mar 2023
EGAF00007947154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74344756
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3679050.494863417131936No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG2832030.3809320458325265Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG1397220.18793793606639855No Hit
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACTCTC957080.1287353744223735TruSeq Adapter, Index 18 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACTCTCAA899090.12093522776508944TruSeq Adapter, Index 18 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACTCTCA850440.11439139029523482TruSeq Adapter, Index 18 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)