FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76356363
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG33058234.329466294773626Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTT21734882.846505405188039TruSeq Adapter, Index 19 (97% over 37bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCT20376602.6686184620920197TruSeq Adapter, Index 19 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTA13309891.7431278124129614TruSeq Adapter, Index 19 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTT11980841.5690689720252913TruSeq Adapter, Index 19 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTC9562951.252410359042376TruSeq Adapter, Index 19 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTG7837091.0263833545869647TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTAC6770890.8867486263063629TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTA5897370.7723482062654031TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTT5669670.7425275088076156TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCT5601330.7335773706246328TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTAT5317200.6963663264055675TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTAA5202280.6813158452819446TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTC4574440.599090870789642TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCA4525940.5927390753276187TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCC4050420.5304626675317157TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTGC4045390.5298039143116338TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTGA3528190.4620688913640373TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTG3320190.4348282015475252TruSeq Adapter, Index 19 (97% over 37bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGA3318530.4346107998884127TruSeq Adapter, Index 19 (100% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTAG3151080.4126807349375716TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTGT3010440.394261837746253TruSeq Adapter, Index 19 (97% over 37bp)
GACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAG2325880.3046085366847554TruSeq Adapter, Index 19 (97% over 37bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGC2165010.2835402204790713TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTACT2004960.26257929545439457TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTGG1720860.2253721801809759TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCTT1610180.2108769900420742TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTCT1606710.210422541995616TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCG1606310.2103701560536612TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTACC1597760.20925040654437665TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTAAC1543870.20219271051451207TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTAC1516100.1985558164942979TruSeq Adapter, Index 19 (97% over 37bp)
GGATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTT1471020.1926519208359885TruSeq Adapter, Index 19 (97% over 37bp)
GGATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTA1462040.19147585643910253TruSeq Adapter, Index 19 (97% over 37bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1448230.18966723179311198Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTTC1347350.17645549723210363TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCTA1297550.16993344745872718TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTTT1283760.16812744210983438TruSeq Adapter, Index 19 (97% over 37bp)
CAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1273110.166732666405287Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTAT1253510.1641657552495003TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCCC1237340.16204805354597626TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTATT1211730.1586940436123182TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCAT1209560.15840984987721324TruSeq Adapter, Index 19 (97% over 37bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1194940.1564951436987642Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTGCT1183320.15497333208497635TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCCT1164420.15249809632761058TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTACA1160940.15204233863260355TruSeq Adapter, Index 19 (97% over 37bp)
GAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT1151970.15086758388426647TruSeq Adapter, Index 1 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCAC1149360.1505257656130112TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTATC1143650.14977795629160598TruSeq Adapter, Index 19 (97% over 37bp)
CAAGCAGAAGACGGATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT1125960.14746118800865357Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTC1122300.14698185663976687TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTTA1087970.14248583317149352TruSeq Adapter, Index 19 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCCG1079400.14136346436511127Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTAAT1062210.1391121785096024TruSeq Adapter, Index 19 (97% over 37bp)
CAAGCAGAAGAGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT1048290.1372891477295743Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCT1042830.13657407962189083TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTAA1034170.13543992397856874TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCTC1002630.13130929245543035TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTGCC998420.13075793041635572TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTGAC974310.1276003677650283TruSeq Adapter, Index 19 (97% over 37bp)
CAAGAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT954750.12503869520343708Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTATA953520.12487760843192597TruSeq Adapter, Index 19 (97% over 37bp)
AAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTG946560.12396609304191192TruSeq Adapter, Index 15 (97% over 36bp)
CATACGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGC944210.12365832563292728TruSeq Adapter, Index 19 (97% over 37bp)
GGCATAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTA908660.11900252504169168TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTCA892980.1169489961170623TruSeq Adapter, Index 19 (97% over 37bp)
GGATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTG884990.11590258692651455TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTCC879110.11513251357977855TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCCA863670.11311041622032207TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTAAA834750.1093229126169878TruSeq Adapter, Index 19 (97% over 37bp)
GGCATAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTT828920.10855938751299612TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTAGC823090.10779586240900448TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCAA802830.10514251444899228TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTGC797510.10444578142099303TruSeq Adapter, Index 19 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)