FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76356363
Sequences flagged as poor quality0
Sequence length151
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56934007.456353048140859No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG26550573.4771915472191885Illumina Single End PCR Primer 1 (100% over 30bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAAGTGTAGAT6489990.849960598568583Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAA5825070.7628794472570675Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAAG4482710.5870774646508503Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAAGT4197020.5496621152581612Illumina Single End PCR Primer 1 (97% over 35bp)
CGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAAGTGTAG3918510.5131870935235614Illumina Single End PCR Primer 1 (97% over 35bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAAGTGTAGATC3666990.4802468132223636Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAAGTG3589530.4701022755628107Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAAGTGT3174020.4156850687086811Illumina Single End PCR Primer 1 (97% over 35bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAAGTGTAGA3084350.40394145017095695Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGGG1607990.21059017700987148Illumina Single End PCR Primer 1 (96% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGG1486590.1946910436265803No Hit
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGAT1393840.18254405333580384Illumina Single End PCR Primer 1 (97% over 35bp)
CGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAAGTGTA1280020.16763763355255673Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG1248470.16350569238086943No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT1163430.15236844112127237No Hit
CTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAAGTGTAGA1133540.14845390160869765Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATC1113930.14588568080436204Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG1081250.14160574934665235No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGG1058380.1386105831153849No Hit
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG850090.11133191349095556Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAAGT798620.10459115240991769Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)