FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71311844
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG18203122.5526082315302348Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT5450890.7643737273151988Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATGCC5233740.7339229651669084TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATGCT4456100.6248751609900874TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATGCA3935280.5518410097486751TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATACC3522260.4939235619822143TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATGTC3311490.4643674618763189TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATCCC3272750.4589349842082333TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATTCT3267540.4582043902833308TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATACT3158270.44288154994281176TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATCCT3053540.4281953499898278TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATCTC3048170.4274423193992852TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATGAC2851320.39983820920407TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATTCC2779620.3897837784141439TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATTGC2741900.3844943344895134TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATTTC2535050.355487932691798TruSeq Adapter, Index 4 (97% over 37bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT2474090.34693956308295715Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATTAC2463660.34547697294154955TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATGTT2322720.3257130750959125TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATACA2187300.30672324221485564TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATATC2184280.3062997501509006TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATGCG2162750.3032806163307178TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATGAT2146260.30096823747819507TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATCCA2099550.2944181334029169TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATTTT2096480.2939876298809494TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATCAC2092510.2934309201147568TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATAAC2090880.29320234658354927TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATTTA1999310.28036156237945553TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATCTT1956200.2743162832810774TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATGTA1938160.27178654923016715TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATAGC1836070.25747055426024323TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATCTA1779120.24948450358400492TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATGAA1768240.24795881032048478TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATCGC1766340.24769237491600976TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATATT1748140.2451402041994595TruSeq Adapter, Index 4 (97% over 37bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCAT1723490.24168355539929665TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATTAT1691090.23714013060719621TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATTAA1649150.2312589196263106TruSeq Adapter, Index 4 (97% over 37bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGC1600900.22449286264424745TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATTCA1592230.22327707582487982TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATAAT1557150.21835783688330931TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATGGC1519130.21302632421060377TruSeq Adapter, Index 4 (97% over 37bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCA1470370.20618875035681308TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATATA1441620.2021571619996252TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATTGT1439680.20188511742874016TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATCAT1409040.19758849595867975TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATCAA1384400.1941332494501194TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATGTG1377780.1932049324092643TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATAAA1312000.18398065824801837TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATTTG1282150.17979481781455547TruSeq Adapter, Index 4 (97% over 37bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1245460.17464980992498244Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA1204840.16895370143562688TruSeq Adapter, Index 2 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATTGA1196580.16779540857196176TruSeq Adapter, Index 4 (97% over 37bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC1127610.15812380338951829TruSeq Adapter, Index 4 (100% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATCTG1066730.14958665211349745TruSeq Adapter, Index 4 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1053140.14768093782569974No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATATG1047800.14693211410996468TruSeq Adapter, Index 4 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATG1024460.14365916550972935TruSeq Adapter, Index 4 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATAA1021830.14329036281827182TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATAGT1006370.1411224200008066TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATAGA986790.13837673304311132TruSeq Adapter, Index 4 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATGC967210.13563104608541604TruSeq Adapter, Index 4 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATA966260.13549782838317853TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATGAG943870.13235809748518074TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATACG920120.1290276549292429TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATGGA910700.12770669623968775TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATCGT908630.12741642187797023TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATGGT891820.12505916969416747TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATTCG876010.12284214667061476TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATCGA847980.11891152330880687TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATCCG843540.11828890583729683TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATTAG838620.11759897836886675TruSeq Adapter, Index 4 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATGA827540.11604523927329659TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATAAG811030.11373005583756884TruSeq Adapter, Index 4 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATAC805990.11302330086990879TruSeq Adapter, Index 4 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATT793760.11130829824005112TruSeq Adapter, Index 4 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACGCATTA724950.10165912972324766TruSeq Adapter, Index 4 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)