FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63271984
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG5203270.8223655512367053Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1560160.24657990809961008No Hit
AATGATACGGCGACCACCGAGATCTACACTGGAGTTGACACTCTTTCCCT1530990.2419696527929328Illumina Single End PCR Primer 1 (96% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCCAT1013810.16023047420166245TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCCATGTGC875240.13832978589702513TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCCATGCTC780670.12338320227164047TruSeq Adapter, Index 9 (97% over 37bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCCA725740.114701634770928TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCCATGACT708070.11190892955087357TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCCATGTAC702150.11097328637584686TruSeq Adapter, Index 9 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCCATGT637470.10075075249734541TruSeq Adapter, Index 9 (97% over 37bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCCATG633230.10008062968279927TruSeq Adapter, Index 9 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)