FastQCFastQC Report
Mon 13 May 2024
EGAF00007947171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63271984
Total Bases9.5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57711159.121122233183016No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG5115370.8084731466615619Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGG2115800.33439760637188176No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGG1782850.28177558016830956No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGGGGG1280020.20230438798947728No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAACTCCAGTGTAGATCT1167730.18455719675235727Illumina Single End Sequencing Primer (100% over 32bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGG1139750.18013501836768703No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGG1128500.17835698023946903No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGG1056860.16703443343897673No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGG1039900.16435394218079205No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA863110.1364126656752221No Hit
GAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAACTCCAGTGTAG841270.13296090098897484Illumina Single End Sequencing Primer (100% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGGG764350.12080386162697221No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGG688390.10879854818524419No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAACTCCAGTGTAGATC680270.10751519977625484Illumina Single End Sequencing Primer (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)