FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58587522
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG5575030.9515729304953365Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2712010.4628989087471561No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGGCAA1285540.21942214931022344TruSeq Adapter, Index 19 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGGCTA1181820.20171872092490956TruSeq Adapter, Index 19 (97% over 38bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGG1125050.19202894432025988TruSeq Adapter, Index 19 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGGCA1023730.17473515947644963TruSeq Adapter, Index 19 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGGCT1008270.17209637232993058TruSeq Adapter, Index 19 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGGCAT982360.16767392892978134TruSeq Adapter, Index 19 (97% over 38bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAG929770.15869761482658373TruSeq Adapter, Index 19 (97% over 38bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGGC889270.1517848800637105TruSeq Adapter, Index 19 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGGCTC812030.138601185419653TruSeq Adapter, Index 19 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGGCTCT787120.13434942682846357TruSeq Adapter, Index 19 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGGCAC777630.13272962799143478TruSeq Adapter, Index 19 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGGCTCA732970.12510684442328862TruSeq Adapter, Index 19 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGGCTT727500.12417319851827835TruSeq Adapter, Index 19 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGGCCA686470.1171700008066564TruSeq Adapter, Index 19 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGGCTAC640910.10939360091044643TruSeq Adapter, Index 19 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGGCACT634150.10823977160187795TruSeq Adapter, Index 19 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGGCTAA616680.10525790798934968TruSeq Adapter, Index 19 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGGCTAT601420.10265325780462263TruSeq Adapter, Index 19 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)