FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52280733
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG3632630.6948314974849339Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCTTA1267650.24246982153062008TruSeq Adapter, Index 5 (97% over 38bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCT870810.16656422931178108TruSeq Adapter, Index 5 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCTTAA821760.15718218793910177TruSeq Adapter, Index 5 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCTTT806840.15432836414133674TruSeq Adapter, Index 5 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA785150.15017960823158313No Hit
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCTT779300.1490606491687865TruSeq Adapter, Index 5 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCTTAT573500.10969624316476206TruSeq Adapter, Index 5 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCTTAC572010.1094112433350925TruSeq Adapter, Index 5 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCTTTA571800.10937107557386389TruSeq Adapter, Index 5 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCTTG563080.10770315710760979TruSeq Adapter, Index 5 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCTTC525740.10056094661105841TruSeq Adapter, Index 5 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)