FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72237108
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG6227620.8621081563785749Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGAC3564560.4934527556114234TruSeq Adapter, Index 4 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACG2800050.3876193382492555TruSeq Adapter, Index 4 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACA2696760.37332059306693177TruSeq Adapter, Index 4 (97% over 39bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2403130.33267250953623445No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACC2013350.2787140924855408TruSeq Adapter, Index 4 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACT1980520.27416933690091805TruSeq Adapter, Index 4 (97% over 39bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGA1529930.2117928087597305TruSeq Adapter, Index 4 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACGC1504310.20824615514785003TruSeq Adapter, Index 4 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACCC1422770.19695832784446468TruSeq Adapter, Index 4 (97% over 39bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGAG1340590.1855819034172852TruSeq Adapter, Index 4 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACAC1334610.18475407404183458TruSeq Adapter, Index 4 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGAGC1295400.17932611587938985TruSeq Adapter, Index 4 (97% over 39bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGAC1281930.17746142328953698TruSeq Adapter, Index 4 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACGT1252260.1733541160036473TruSeq Adapter, Index 4 (97% over 39bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA1235100.17097860562191944No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACGA1194270.16532638598987104TruSeq Adapter, Index 4 (97% over 39bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAG1180910.16347692103067027TruSeq Adapter, Index 4 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACTC1156540.16010330867620007TruSeq Adapter, Index 4 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACCT1124400.1556540718656677TruSeq Adapter, Index 4 (97% over 39bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGAT1074980.14881271271269608TruSeq Adapter, Index 4 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGAGT1040590.1440520016388253TruSeq Adapter, Index 4 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACGG1018040.14093033735514437TruSeq Adapter, Index 4 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGATA955900.1323281103667661TruSeq Adapter, Index 4 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACTT941380.1303180631206886TruSeq Adapter, Index 4 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGATC908130.12571516567357596TruSeq Adapter, Index 4 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGAGG881470.12202454173553016TruSeq Adapter, Index 4 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGAGA879800.12179335861563007TruSeq Adapter, Index 4 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACTA848080.11740226366758758TruSeq Adapter, Index 4 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACAT846580.11719461415869528TruSeq Adapter, Index 4 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACCA835710.11568984738425574TruSeq Adapter, Index 4 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACAA821960.113786393552743TruSeq Adapter, Index 4 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGATT802600.11110633055797306TruSeq Adapter, Index 4 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGAGCT751140.10398256807290791TruSeq Adapter, Index 4 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)