FastQCFastQC Report
Mon 13 May 2024
EGAF00007947189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64295241
Total Bases9.7 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9574801.4891926449113084No Hit
ATTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTGAAGTGTA5781340.8991863021401538Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG997890.15520433308586556Illumina Single End PCR Primer 1 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA957160.14886949408899486No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGG669130.10407146619140908No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)