FastQCFastQC Report
Sun 5 May 2024
EGAF00007947198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43492340
Total Bases6.5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG11889272.73364689046393Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTAC3688980.8481907388749375TruSeq Adapter, Index 25 (97% over 37bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTG3468750.7975542359873026TruSeq Adapter, Index 25 (97% over 37bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGT3337790.7674431865473322TruSeq Adapter, Index 25 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTA2839800.6529425641388805TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTAA2532970.5823945090100924TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCT2329000.5354965954924475TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCA2202380.5063834229199901TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCC2178460.5008836038713943TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTGC2175470.50019612649032TruSeq Adapter, Index 25 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTC2142490.49261318199940496TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTA2085270.47945684228533114TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTAT1970180.45299471125260216TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTC1944580.44710861728754997TruSeq Adapter, Index 25 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTT1819610.41837482186518365TruSeq Adapter, Index 25 (97% over 37bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA1797710.4133394524185178No Hit
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTG1696400.3900456954029146TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTT1694830.3896847122964642TruSeq Adapter, Index 25 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1668010.3835181091658899No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTACT1645210.3782758067282652TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTACC1347770.3098867524718146TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCTC1334600.3068586330374498TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTGA1332100.3062838191736752TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCCT1287660.29606592793121733TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCCC1224010.2814311669595152TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTGCC1152260.2649340090691832TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTCT1040550.23924902638027754TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTACA1021460.23485974771649443TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTGCT1002140.23041758617724412TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTG993560.22844482499676955TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTAAC967900.22254493549898674TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTAG907140.20857465935380806TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCTT904540.20797685293548243TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCAC894450.205656904181288TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCCA861810.1981521343758464TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTAC839610.19304778726552768TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCTA831140.19110031789505924TruSeq Adapter, Index 25 (97% over 37bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTT827310.1902197030557565TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTCC812740.18686968785767794TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCAA805970.18531309191457623TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCAT801620.18431291579160836TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTGCA791110.18189639830829982TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTCA781490.17968451456049503TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTTC772700.17766346901546343TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTATC726030.16693284380651857TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCG692170.15914756483555495TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTAA692150.15914296632464475TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTGT692060.15912227302554888TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTAAT682890.15701385577322352TruSeq Adapter, Index 25 (97% over 37bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTG681990.15680692278226466TruSeq Adapter, Index 25 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTT659680.15167728386193982TruSeq Adapter, Index 6 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTGAC658020.15129560745639348TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTTA620430.1426527062006781TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTAAA619270.14238599256788667TruSeq Adapter, Index 25 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTA613040.14095355641936028TruSeq Adapter, Index 6 (96% over 33bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTC599870.13792543698499551TruSeq Adapter, Index 6 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTGC597510.13738281269759228TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTATT585000.134506444123264TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTTT578090.13291765860379093TruSeq Adapter, Index 25 (97% over 37bp)
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA551850.12688441228961236No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTAT541340.12446789480630381TruSeq Adapter, Index 25 (97% over 37bp)
CAAGCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGT539250.12398735041618822TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTATA527320.1212443386582557TruSeq Adapter, Index 25 (97% over 37bp)
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC501670.11534674841592796No Hit
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG489800.11261753219072601Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTGG470920.10827653789149998TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTAGC440870.1013672752489289TruSeq Adapter, Index 25 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)