FastQCFastQC Report
Sun 5 May 2024
EGAF00007947199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43492340
Total Bases6.5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG9759422.2439399673597697Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5491311.262592447313711No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTATGCGGTGTGTAGATC4581791.0534705651615894Illumina Single End Sequencing Primer (100% over 33bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA1230420.28290498970623335No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1040930.2393363980875713No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGG932730.2144584540634052No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGGG636530.14635450748338674Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTATGCGGTGTGT554050.12739024848973404Illumina Single End Sequencing Primer (100% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGG497180.11431438271658871No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGG485660.11166564043231522No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG469950.10805351011235541No Hit
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTATGCGGT465210.10696366302663872Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTATGCGGTGTG449120.10326416099938518Illumina Single End Sequencing Primer (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)