FastQCFastQC Report
Mon 13 May 2024
EGAF00007947202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72478231
Total Bases10.9 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG2104390.29034787010737056Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT917610.12660491120430353No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC771750.10648024784158984No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC770740.10634089565458628No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)