FastQCFastQC Report
Mon 13 May 2024
EGAF00007947208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79526336
Total Bases12 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG4354140.547509192426519Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1514730.1904689787292602No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTCTTCTCT1155750.14532921521745953TruSeq Adapter, Index 16 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTCTTCTGC950190.11948117413582338TruSeq Adapter, Index 16 (97% over 38bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA899530.11311095735631527No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTCTTCAA826060.10387250834742341TruSeq Adapter, Index 16 (97% over 38bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTCT808130.10161790931748699TruSeq Adapter, Index 16 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTCTTCTCA799240.10050004064062501TruSeq Adapter, Index 16 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)