FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44630453
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG24003475.378271647836512Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAA3718320.8331351689394683TruSeq Adapter, Index 8 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTA2722420.6099915678651078TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCTA2521470.5649662574565398TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCAA2476130.5548072747547509TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCA2436150.5458492657468658TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCAT2353210.5272655422072458TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCTT2287810.5126118706435715TruSeq Adapter, Index 8 (97% over 37bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCT2201030.4931677480396625TruSeq Adapter, Index 8 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTC2078380.4656865123013652TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTACT2051900.45975334375387134TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTCT2025780.4539008376186547TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTA2024560.4536274816659378TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAT1970030.44140936682851956TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCCA1904910.42681843269661635TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCCT1885080.42237527815368575TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAC1800770.40348458932290027TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAAC1767250.3959740224908763TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTACA1727410.38704738219887663TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCAC1684910.3775247363050516TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTTT1634380.3662028704929345TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAAA1609730.360679735874516TruSeq Adapter, Index 8 (97% over 37bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGC1592690.356861715026733TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCT1590770.3564315154945884TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTATT1581230.35429396156924514TruSeq Adapter, Index 8 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTT1559910.34951695426438983TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTACC1554170.34823083691308265TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTAC1536360.3442402881279292TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAAT1484580.3326383444954054TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCTG1455520.3261270953265924TruSeq Adapter, Index 8 (97% over 37bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1454220.32583581439336945Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTTA1439400.3225152117546286TruSeq Adapter, Index 8 (97% over 37bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTT1416150.3173057642950655TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTAA1397470.31312028134690906TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTAT1376400.30839928960613505TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTCA1369180.3067815601154664TruSeq Adapter, Index 8 (97% over 37bp)
AAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCT1348320.30210762144852077TruSeq Adapter, Index 8 (97% over 36bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAG1340150.30027703281434315TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTATA1325640.29702588947506314TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTTC1318490.29542384434233726TruSeq Adapter, Index 8 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1289130.28884537649662667No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCTC1258330.2819442590018076TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTATC1248410.2797215614190606TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCCC1235210.2767639396355668TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTT1226130.2747294543481331TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGCT1213740.2719533229922627TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCGA1206350.27029750291801874TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGA1128570.2528699406210374TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCGT1091190.2444944934795979TruSeq Adapter, Index 8 (97% over 37bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT1063240.2382319534153059Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTCC1041330.2333227493792187TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGC947870.2123818909030567TruSeq Adapter, Index 8 (97% over 37bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC931830.208787932311599Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCAG926350.2075600711469364TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTC921540.20648233169401173TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGAA908110.20347317559156303TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGAC903860.2025209110021805TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCC888580.19909723972553TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAG855220.1916225228545182TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTGC838760.18793445811540385TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGCA836150.18734965562639483TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCCG835480.18719953391465688TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCGC823870.18459817111872023TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCG822020.18418365594451844TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAGC819730.1836705533775335TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTGA802800.17987717937794628TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGAT778530.17443918841693137TruSeq Adapter, Index 8 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTG744560.16682779356956112TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGCC721550.1616721210515161TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAGA715220.16025380696897698TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAAG664680.1489297005342966TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTAG630850.1413496744028119TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCGG626200.1403077849108993TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTTG622100.13938912965996558TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTG599310.13428275083831212TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGTT584370.13093526072881223TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGTA577300.1293511405765924TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTATG561250.12575494136257143TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTGT560430.1255712103123847TruSeq Adapter, Index 8 (97% over 37bp)
CAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC557140.12483404548907448Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAAGCAGAAGACGGCATACGAGAGATCGGAAGAGCACACGTCTGAACTCC553370.1239893307827281Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTACG550840.1234224532742251TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGC517700.11599703009960485TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAGT493980.11068227337956889TruSeq Adapter, Index 8 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT492110.11026327696024057Illumina PCR Primer Index 2 (96% over 31bp)
CAAGCAGAAGACGGATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT489050.10957764645588518Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTCG474030.10621223136587925TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGGC466750.10458105813983111TruSeq Adapter, Index 8 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)