FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62505035
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2774380.44386504223219775No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG1201770.19226771091320885Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT855880.1369297689378144No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTTCTCAAA853090.13648340489690153TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTTCTCTAA806280.12899440821047456TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTTCTCTCT761840.1218845809781564TruSeq Adapter, Index 8 (97% over 37bp)
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG758730.12138702106158329No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC720820.11532190966695724No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTTCTCATA689570.11032231243451028TruSeq Adapter, Index 8 (97% over 37bp)
CGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAA669030.10703617716556754No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTTCTCAAC625720.10010713536917465TruSeq Adapter, Index 8 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)