FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62505035
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16749842.679758518653737No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1974190.3158449555303825No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG1563620.25015904718715865No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1124020.1798287129988808Illumina Single End PCR Primer 1 (100% over 30bp)
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA929840.1487624156997912No Hit
GGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGAACGAAGGTGTA763260.12211176267639878Illumina Single End PCR Primer 1 (96% over 33bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT748080.11968315832476535No Hit
GGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTA666930.10670020423154711No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGG662080.10592426674107135No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)