FastQCFastQC Report
Sat 18 Mar 2023
EGAF00007947218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78666909
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3573100.45420622793250975No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG1589360.20203666575993218Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGGCATAA1132060.14390548890131175TruSeq Adapter, Index 3 (97% over 41bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA943970.11999581679254742No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC796420.10123951863927946No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)