FastQCFastQC Report
Sun 5 May 2024
EGAF00007947250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16288245
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG1878291.1531567704194037Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGT586950.3603518979484899TruSeq Adapter, Index 9 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGTGC360220.2211533532311185TruSeq Adapter, Index 9 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGTGCA299320.1837644264314541TruSeq Adapter, Index 9 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGTGA257910.15834118408705175TruSeq Adapter, Index 9 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGTGCT249670.15328232108492965TruSeq Adapter, Index 9 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGTGT236480.145184456643426TruSeq Adapter, Index 9 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGTGAC235330.1444784260059939TruSeq Adapter, Index 9 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGTGCC230900.14175867320266855TruSeq Adapter, Index 9 (97% over 36bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGTG221040.1357052279112943TruSeq Adapter, Index 9 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGTGG172290.10577566828102107TruSeq Adapter, Index 9 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGTGCG168150.10323395798626554TruSeq Adapter, Index 9 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGTGTC164190.10080275683476028TruSeq Adapter, Index 9 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)