FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16282494
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG642310.39447888020103067Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT211270.12975284990124977No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC199440.12248737816209236No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA182790.11226167195271192No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG177570.10905577487085209No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)