FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16282494
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG743380.4565516805963509Illumina Single End PCR Primer 1 (100% over 30bp)
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG228510.14034090846279446No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT216070.13270080124089098No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACCGATCA170570.10475667916720866No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)