FastQCFastQC Report
Sun 5 May 2024
EGAF00007947256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15795385
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG3113911.9714049388476442Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTGTGT446730.28282311573918584TruSeq Adapter, Index 12 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTGTGTAA295310.1869596720814339TruSeq Adapter, Index 12 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA246760.15622284610346632No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTGTGTAAA243090.15389938263613076TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTGTGTAAAA186530.11809145519403293TruSeq Adapter, Index 12 (97% over 38bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTGTGTA167960.10633485666857756TruSeq Adapter, Index 12 (97% over 38bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTG164440.10410635764813583TruSeq Adapter, Index 12 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)