FastQCFastQC Report
Sun 5 May 2024
EGAF00007947257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15795385
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG3468682.1960085176777904Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG344320.21798772236320926No Hit
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG161140.10201713981647172Illumina Single End PCR Primer 1 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)