FastQCFastQC Report
Sun 5 May 2024
EGAF00007947266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19214511
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG1288920.670805517767275Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA309360.1610033167120412No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGTCGAGAA215510.11216002322411431TruSeq Adapter, Index 15 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGTCGAGAC205930.1071742080763856TruSeq Adapter, Index 15 (97% over 38bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGTCGA195350.10166795293411318TruSeq Adapter, Index 15 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)