FastQCFastQC Report
Fri 10 May 2024
EGAF00007947272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14999473
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG2771901.8479982596721896Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGA289450.1929734464670859TruSeq Adapter, Index 7 (97% over 37bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGACGT280720.1871532419839017TruSeq Adapter, Index 7 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGACGTGAA263660.17577950905341808TruSeq Adapter, Index 7 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGACGTGA225890.1505986243650027TruSeq Adapter, Index 7 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGACGTGTA164350.10957051624413737TruSeq Adapter, Index 7 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGACGTGAAC157750.10517036165203937TruSeq Adapter, Index 7 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)