FastQCFastQC Report
Fri 10 May 2024
EGAF00007947278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15351091
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG600270.39102758233926177Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA463230.3017570542706053No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATACGCGTAC174820.11388115672039205TruSeq Adapter, Index 8 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATACGCGTGC157340.10249434388734976TruSeq Adapter, Index 8 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)