FastQCFastQC Report
Fri 10 May 2024
EGAF00007947283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15072088
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3555582.359049389839019No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG576360.38240222588933925Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG357210.2370010047712036No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCCGATCAGTGT353910.23481152710891814Illumina Single End Sequencing Primer (100% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT247440.16417101598663703No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGT152760.10135291142143012No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)