FastQCFastQC Report
Sat 11 May 2024
EGAF00007947292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15237328
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG1488560.9769166877552286Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCTTA482890.31691251904533396TruSeq Adapter, Index 5 (97% over 38bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCT342060.2244881779797613TruSeq Adapter, Index 5 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCTTAA301050.19757401035142122TruSeq Adapter, Index 5 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCTTT300320.19709492372940976TruSeq Adapter, Index 5 (97% over 38bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCTT297920.19551984442416678TruSeq Adapter, Index 5 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCTTG210090.13787850468271076TruSeq Adapter, Index 5 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA209050.13719597031710545No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCTTAC208140.13659875274720082TruSeq Adapter, Index 5 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCTTTA207280.13603434932948874TruSeq Adapter, Index 5 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCTTAT203320.13343546847583776TruSeq Adapter, Index 5 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCTTC197350.12951745870404574TruSeq Adapter, Index 5 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)