FastQCFastQC Report
Mon 6 May 2024
EGAF00007947294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15978070
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG528060.33049047851211066Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA295150.1847219345014761No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)