FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20341924
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG845860.41582104033030504Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT261550.1285768248863775No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC217590.10696628303202785No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC216680.10651893105096646No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)