FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19083284
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTGGACAGTGTAGATC743900.3898176016245422Illumina Single End PCR Primer 1 (96% over 33bp)
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG449490.23554122026376592No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG366780.19219962350295683No Hit
GGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTGGACAGTGTAG351300.18408781213967157Illumina Single End PCR Primer 1 (96% over 33bp)
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA246910.12938548731968774No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA225980.11841777337695127No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)