FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7550862
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG6767808.96295019032264Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA458340.6070035447608498No Hit
CAAGCAGAAGACGGCATACGAGATTTAGATCGGAAGAGCACACGTCTGAA310140.410734562491011Illumina PCR Primer Index 11 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATTAGATCGGAAGAGCACACGTCTGAAC299430.39655075142414203RNA PCR Primer, Index 29 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT207240.27445873067207427Illumina PCR Primer Index 2 (96% over 31bp)
CAAGCAGAAGACGGCATACGAGATATAGATCGGAAGAGCACACGTCTGAA182830.24213129573815548Illumina PCR Primer Index 6 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATTAAGATCGGAAGAGCACACGTCTGAA160820.2129823058612381RNA PCR Primer, Index 32 (96% over 28bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTAA160640.212743922481963TruSeq Adapter, Index 4 (97% over 37bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCT153780.20365886702736724TruSeq Adapter, Index 4 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATAAGATCGGAAGAGCACACGTCTGAAC149090.19744765564514355Illumina PCR Primer Index 10 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATTCAGATCGGAAGAGCACACGTCTGAA116660.1544989168124116RNA PCR Primer, Index 40 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATGTAGATCGGAAGAGCACACGTCTGAA109850.14548007896317003RNA PCR Primer, Index 39 (96% over 29bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTA108560.14377166474503178TruSeq Adapter, Index 4 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTAT98970.13107112803809684TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTGCT97360.1289389211456917TruSeq Adapter, Index 4 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTA95840.12692590594292413TruSeq Adapter, Index 4 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTTA94080.12459504623445641TruSeq Adapter, Index 4 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTT87700.11614567979126092TruSeq Adapter, Index 4 (96% over 33bp)
CAAGCAGAAGACGGCATACGAGATAAAGATCGGAAGAGCACACGTCTGAA87290.1156026954273565Illumina PCR Primer Index 2 (96% over 27bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTAAA84810.11231830220178834TruSeq Adapter, Index 4 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTGA82770.10961662390333712TruSeq Adapter, Index 4 (97% over 37bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTT82360.10907363953943272TruSeq Adapter, Index 4 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTAAT77920.10319351618398005TruSeq Adapter, Index 4 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTGC77260.10231944379330467TruSeq Adapter, Index 4 (97% over 37bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC76170.10087589999658317Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)