FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7550862
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG6650108.807073947318862Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG418840.5546916365310345No Hit
TATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA389390.5156894669774126Illumina Single End PCR Primer 1 (100% over 25bp)
TAATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG388200.5141134879699828Illumina PCR Primer Index 4 (96% over 26bp)
TTATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG213130.28225916458279865Illumina PCR Primer Index 2 (96% over 26bp)
AAATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG191220.25324260991658964Illumina PCR Primer Index 4 (96% over 26bp)
AATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA189210.250580662181351Illumina Single End PCR Primer 1 (100% over 25bp)
GAATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG168270.2228487290590134Illumina PCR Primer Index 2 (96% over 26bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA144880.1918721332743202No Hit
ATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA143500.19004452736654437Illumina Single End PCR Primer 1 (100% over 26bp)
TTCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA140270.18576687006066325Illumina Single End PCR Primer 1 (100% over 26bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTATGCCTGTGT130320.17258956659517816Illumina Single End PCR Primer 1 (96% over 33bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA127300.16859002323178465No Hit
TCATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG117500.15561137258236213Illumina PCR Primer Index 3 (96% over 26bp)
TGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTATGCCTGTGTAGATC117280.15532001511880364Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTATGCCTGTGTAGATC117200.15521406695023693Illumina Single End PCR Primer 1 (96% over 33bp)
ATATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG112610.1491352907787217Illumina PCR Primer Index 2 (96% over 26bp)
TGATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG102790.13613015308715748Illumina Paired End PCR Primer 2 (96% over 26bp)
CATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA98110.12993218522600467Illumina Single End PCR Primer 1 (100% over 25bp)
GTATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG93840.12427720172875627Illumina PCR Primer Index 2 (100% over 26bp)
GATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA87980.11651649838124442Illumina Single End Adapter 1 (96% over 25bp)
ACATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG79040.10467679054391406Illumina PCR Primer Index 3 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)