FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7144991
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG98255813.751703816002008Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1528862.139764766673604No Hit
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA194780.27261056032120967No Hit
GGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA174790.24463291836196854No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACA116210.1626454113098253TruSeq Adapter, Index 13 (97% over 35bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT113460.1587965611153324Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT112650.15766289978531814Illumina PCR Primer Index 2 (96% over 31bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCA109710.153548129031933TruSeq Adapter, Index 13 (97% over 37bp)
GGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG103340.14463279239959856No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA100350.14044804255176807No Hit
CCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC98840.13833467389951926No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACTCT89740.12559847871047003TruSeq Adapter, Index 13 (97% over 37bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC86270.12074192955596444Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACT79400.11112680197917675TruSeq Adapter, Index 13 (97% over 35bp)
GCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC74690.10453477128242708No Hit
ACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC74370.104086905077977No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)